Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10819003 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2011 | 10 Pages |
Abstract
The swimming crabs Callinectes danae and C. ornatus are found in bays and estuaries, but C. danae is more abundant in lower salinities, while C. ornatus remains restricted to areas of higher salinity. Experimental crabs of both species were submitted to: air exposure (Ae, 3Â h), reimmersion in 33â° (control) sea water (SW) (Ri, 1Â h) following air exposure; hyposaline (Ho, 10â° for 2Â h) or hypersaline (He, 40â° for 2Â h) SW, then return to control 33â° SW (RHo and RHe, for 1Â h). Hemolymph was sampled for osmolality and chloride determinations. Activity of antioxidant enzymes [glutathione peroxidase (GPX), catalase, glutathione-S-transferase] and levels of carbonyl proteins and lipid peroxidation (TBARS) were evaluated in hepatopancreas, muscle, anterior and posterior gills. In Ho groups, hemolymph concentrations were lower in both species, compared to He groups. C. danae displayed higher control activities of GPX (hepatopancreas and muscle) and catalase (all four tissues) than C. ornatus. C. ornatus presented increased activities of catalase and GPX in Ae, Ri, and He groups. Increased TBARS was seen in C. ornatus tissues (He group). The more euryhaline species displayed higher constitutive activities of antioxidant enzymes, and the less euryhaline species exhibited activation of these enzymes when exposed to air or hyper-salinity.
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Authors
Carolina A. Freire, Valéria G. Togni, Marcelo Hermes-Lima,