Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10819010 | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology | 2011 | 9 Pages |
Abstract
The effects of fish oil (FO) substitution by 66% vegetable oils in a diet with already 75% vegetable protein (66VO) on adipose tissue lipid metabolism of gilthead sea bream were analysed after a 14-month feeding trial. In the last 3 months of the experiment, a FO diet was administrated to a 66VO group (group 66VO/FO) as a finishing diet. Hormone-sensitive lipase (HSL) activity was measured in adipose tissue and adipocyte size, and HSL, lipoprotein lipase and liver X receptor gene expression in isolated adipocytes, on which lipolysis and glucose uptake experiments were also performed. Lipolysis was measured after incubation with tumour necrosis factor-α (TNFα), linoleic acid, and two conjugated linoleic acid isomers. Glucose uptake was analysed after TNFα or insulin administration. Our results show that FO replacement increased lipolytic activity and adipocyte cell size. The higher proportion of large cells observed in the 66VO group could be involved in their observed lower response to fatty acid treatments and lower insulin sensitivity. The 66VO/FO group showed a moderate return to the FO conditions. Therefore, FO replacement can affect the morphology and metabolism of gilthead sea bream adipocytes which could potentially affect other organs such as the liver.
Keywords
TNFαc9,t11-CLAT10MFIHSLLXRLPLdpmCLAHSIBSAbovine serum albuminLinoleic acidPolyunsaturated fatty acidsPUFAnon-esterified fatty acidsNEFAsdisintegrations per minutetumour necrosis factor-αGlucose uptakeVegetable oilFish oilVegetable oilsHepatosomatic indexhormone-sensitive lipaseLipoprotein lipaseLipolysisrelative unitsliver X receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Lourdes Cruz-Garcia, Joan Sánchez-Gurmaches, Lamia Bouraoui, Alfonso Saera-Vila, Jaume Pérez-Sánchez, Joaquim Gutiérrez, Isabel Navarro,