| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10824127 | Insect Biochemistry and Molecular Biology | 2013 | 9 Pages |
Abstract
⺠We cloned glucosidase II subunit genes from Bombyx mori (BmGlcIIα and BmGlcIIβ) and Spodoptera frugiperda (SfGlcIIα). ⺠Recombinant glucosidase II subunits (rBmGlcIIα, rBmGlcIIβ, and rSfGlcIIα) were produced by a baculovirus expression system. ⺠rBmGlcIIα and rSfGlcIIα hydrolyzed p-nitrophenyl α-d-glucopyranoside but not N-glycan. ⺠The formation of a rBmGlcIIα/rBmGlcIIβ complex was required for N-glycan cleavage. ⺠rBmGlcIIα was less sensitive than rSfGlcIIα to α-glucosidase inhibitors in mulberry leaves.
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Authors
Satoko Watanabe, Akemi Kakudo, Masato Ohta, Kazuei Mita, Kazuhito Fujiyama, Shigeki Inumaru,