| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10824151 | Insect Biochemistry and Molecular Biology | 2012 | 9 Pages |
Abstract
⺠Musca domestica larvae have 19 putatively functional chymotrypsins differentially expressed along the midgut. ⺠The major chymotrypsin activity was purified from the midgut and shown to prefer long substrates rather than short ones. ⺠The most transcribed chymotrypsin gene was cloned and expressed. ⺠The enzyme was immunolocalized inside vesicles (denoting exocytosis) and midgut lúmen, indicating luminal action. ⺠Drosophila melanogaster and M. domestica chymotrypsin genes expanded before evolutionary separation of the genera.
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Authors
Fábio K. Tamaki, Marcelo H.P. Padilha, Andre C. Pimentel, Alberto F. Ribeiro, Walter R. Terra,