The characterization of Lucilia cuprina acetylcholinesterase as a drug target, and the identification of novel inhibitors by high throughput screening

► L. cuprina AChE is a GPI-anchored cell-surface enzyme. ► Characterization of L. cuprina AChE as pesticide drug target. ► High throughput inhibitor screening of >100,000 compounds. ► Identification of highly potent and selective non-OP, non-CB inhibitors. ►Highly L. cuprina AChE-selective hits are no or poor inhibitors of cat flea AChE.