| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10840374 | Plant Physiology and Biochemistry | 2011 | 10 Pages |
Abstract
⺠A tomato chloroplast glutathione reductase gene was cloned. ⺠We obtained antisense transgenic tomato lines with 60% decrease in GR activity. ⺠Transgenic plants displayed accumulation of H2O2 and improved sensitivity to chilling. ⺠Decrease in GR activity affected the activities of antioxidative enzymes APX and SOD. ⺠GR is essential for glutathione-ascorbate cycle and ROS scavenge.
Keywords
DTNBNADPHN-ethylmaleimideGSHPFDDHARdehydroascorbate reductaseGSSGrecAPXdehydroascorbatemonodehydroascorbate reductasethe maximal photochemical efficiency of PSIIMDAMonodehydroascorbate radical5,5-dithiobis-2-nitrobenzoic acidMDARβ-nicotinamide adenine dinucleotide 2′-phosphateDTTGFPASATBAORFFv/FmROSAscorbateEDTAEthylenediaminetetraacetic acidChilling stressThiobarbituric acidphoton flux densityrapid amplification of cDNA endsDHAdithiothreitolSODSuperoxide dismutaseopen reading frameMethyl viologenRaceNEMwild typeascorbate peroxidaseGreen fluorescence proteinglutathione reductaseTomatoReactive oxidative speciesReactive oxygen species
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Authors
De-Feng Shu, Li-Yan Wang, Ming Duan, Yong-Sheng Deng, Qing-Wei Meng,