Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10840946 | Plant Science | 2005 | 8 Pages |
Abstract
Vernalization is induced by prolonged cold and accelerates flowering. To monitor the genome-wide transcriptome change that identifies the genes that are differently up-regulated depending on the duration of cold exposure, we fabricated a Chinese cabbage cDNA microarray and used it to identify genes that are up-regulated by short-term (1-week) and long-term (4-week) cold. The overall expression patterns between 1- and 4-week cold treated seedlings were very similar but some genes in the cell growth/division/DNA synthesis functional category were regulated differently. Prolonged cold specifically up-regulated several heat shock proteins (HSPs), and small HSPs were more significant. Among the up-regulated sHSPs, we chose the small HSP 17.6-II and confirmed its up-regulation in response to long- but not short-term cold exposure by Northern analysis. And its constitutive overexpression moderately accelerated flowering.
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Authors
Kyung Ae Yang, Chan Ju Lim, Joon Ki Hong, Zheng Lu Jin, Jong Chan Hong, Dae-Jin Yun, Woo Sik Chung, Sang Yeol Lee, Moo Je Cho, Chae Oh Lim,