Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10869877 | FEBS Letters | 2015 | 30 Pages |
Abstract
Clostridium thermocellum efficiently degrades crystalline cellulose by a high molecular weight protein complex, the cellulosome. The bacterium regulates its cellulosomal genes using a unique extracellular biomass-sensing mechanism that involves alternative sigma factors and extracellular carbohydrate-binding modules attached to intracellular anti-sigma domains. In this study, we identified three cellulosomal xylanase genes that are regulated by the ÏI6/RsgI6 system by utilizing sigI6 and rsgI6 knockout mutants together with primer extension analysis. Our results indicate that cellulosomal genes are expressed from both alternative ÏI6 and ÏA vegetative promoters.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Andy Sand, Evert K. Holwerda, Natalie M. Ruppertsberger, Marybeth Maloney, Daniel G. Olson, Yakir Nataf, Ilya Borovok, Abraham L. Sonenshein, Edward A. Bayer, Raphael Lamed, Lee R. Lynd, Yuval Shoham,