Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10869926 | FEBS Letters | 2015 | 8 Pages |
Abstract
Impaired intestinal barrier function is one of the critical issues in inflammatory bowel diseases. The aim of this study is to investigate muscarinic cholinoceptor (mAChR)-mediated signaling for the amelioration of cytokine-induced barrier dysfunction in intestinal epithelium. Rat colon challenged with TNF-α and interferon γ reduced transepithelial electrical resistance (TER). This barrier injury was attenuated by muscarinic stimulation. In HT-29/B6 intestinal epithelial cells, muscarinic stimulation suppressed TNF-α-induced activation of NF-κB signaling and barrier disruption. Finally, muscarinic stimulation promoted the shedding of TNFR1, which would be a mechanism for the attenuation of TNF-α/NF-κB signaling and barrier disruption via mAChR.
Keywords
IFN γATRNMSMT-3IBDmAChRMT-7FD-4CCHMuscarinic cholinoceptorKHsKrebs–Henseleit solutionFBSAtropineIntestinal epitheliuminterferon γInflammatory bowel disease (IBD)inflammatory bowel diseasesTERfetal bovine serumintestinal epithelial cellTumor necrosis factor (TNF)Intestinal barriertransepithelial electrical resistanceCarbacholIECmuscarinic acetylcholine receptor
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Authors
Md Rafiqul Islam Khan, Junsuke Uwada, Takashi Yazawa, Md Tariqul Islam, Susanne M. Krug, Michael Fromm, Shin-ichiro Karaki, Yuichi Suzuki, Atsukazu Kuwahara, Hatsumi Yoshiki, Kiyonao Sada, Ikunobu Muramatsu, Abu Syed Md Anisuzzaman, Takanobu Taniguchi,