Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10869994 | FEBS Letters | 2015 | 7 Pages |
Abstract
During infection with human cytomegalovirus (HCMV), overexpression of hcmv-miR-US33 can inhibit the lytic viral replication and down-regulate US29 mRNA. However, it remains unknown whether inhibition of viral replication by miR-US33 is mediated by down-regulation of expression of US29 or another host gene. Here, we identified the host gene Syntaxin3 (STX3) to be a direct target of hcmv-miR-US33-5p using Hybrid-PCR and luciferase-reporter assays. It was further demonstrated that the levels of STX3 protein were down-regulated in hcmv-miR-US33-5p-overexpressing cells. Experiments with STX3-specific siRNA, or with an inhibitor of hcmv-miR-US33-5p confirmed that hcmv-miR-US33-5p-mediated inhibition of HCMV DNA synthesis and of viral replication are specifically mediated by down-regulation of STX3 expression.
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Authors
Xin Guo, Ying Qi, Yujing Huang, Zhongyang Liu, Yanping Ma, Yaozhong Shao, Shujuan Jiang, Zhengrong Sun, Qiang Ruan,