Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870008 | FEBS Letters | 2015 | 8 Pages |
Abstract
TCAM1P is a unitary pseudogene, which was disabled since the human-mouse divergence. Here we found that TCAM1P was specifically expressed in the human testis, with different cell type-specificity from mouse Tcam1, and characterized its transcripts. At the mouse locus, a multifunctional dual promoter-enhancer (DPE) controls the expression of Tcam1 and Smarcd2 genes. The corresponding human sequence was found to potentially function as a DPE, although the molecular mechanism was different from mouse. Interestingly, the change in DPE activity occurred before pseudogenization of TCAM1P. These data suggest the presence of a DPE in the human genome for the first time, and provide an important model of evolutionary changes in the regulatory mechanism of a pseudogene.
Keywords
LCRlncRNATSSORFTTSIGVRT-PCRDPEhGHlong non-coding RNAHumanTestisanalysis of varianceANOVArapid amplification of cDNA endsCNSconserved non-coding sequenceTranscriptional start siteopen reading frameIntegrative Genomics ViewerRacelocus control regionHuman growth hormonereverse transcription-polymerase chain reaction
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Authors
Misuzu Kurihara, Atsushi P. Kimura,