| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10870047 | FEBS Letters | 2014 | 8 Pages |
Abstract
Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Golgi BIG/Sec7-subfamily, hypB. Both geaA and hypB are essential. hypB5 conditionally blocks secretion. We sought extragenic hypB5 suppressors and obtained geaA1. geaA1 results in Tyr1022Cys within a conserved GBF/Gea-specific S(Y/W/F)(L/I) motif in GeaA. This mutation alters GeaA localization. Remarkably, geaA1 suppresses hypBÎ, indicating that a single mutant Golgi Arf1-GEF suffices for growth.
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Authors
Herbert N. Jr., Miguel Hernandez-Gonzalez, Miguel A. Peñalva, Areti Pantazopoulou,