Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870079 | FEBS Letters | 2015 | 4 Pages |
Abstract
PixD is a blue light using flavin (BLUF)-type blue-light photoreceptor controlling phototaxis in the cyanobacterium Synechocystis sp. PCC6803. The crystal structure of PixD shows a decamer, although in solution an equilibrium is maintained between the dimer and decamer. Because the ratio of these two conformers is altered by illumination, the equilibrium state determines photosensitivity. However, no structural information is available for the PixD dimer. Here, we report a predicted structure for the dimer based on docking simulation, mutagenesis, and mass spectrometry-based cross-linking analyses. The results indicate the importance of the PixD C-terminus for dimer preference and photosensitivity.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Shukun Ren, Yuki Sugimoto, Taichi Kobayashi, Shinji Masuda,