Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870161 | FEBS Letters | 2014 | 29 Pages |
Abstract
Human mesenchymal stem cells (hMSCs) have self-renewal and differentiation capabilities but the regulatory mechanisms of MSC fate determination remain poorly understood. Here, we aimed to identify microRNAs enriched in hMSCs that modulate differentiation commitments. Microarray analysis revealed that miR-140-5p is commonly enriched in undifferentiated hMSCs from various tissue sources. Moreover, bioinformatic analysis and luciferase reporter assay validated that miR-140-5p directly represses bone morphogenic protein 2 (BMP2). Furthermore, blocking miR-140-5p in hMSCs increased the expression of BMP signaling components and critical regulators of osteogenic differentiation. We propose that miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSCs.
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Authors
Supyong Hwang, Seul-Ki Park, Ha Yeon Lee, Seong Who Kim, Jung Shin Lee, Eun Kyung Choi, Dalsan You, Choung-Soo Kim, Nayoung Suh,