Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870239 | FEBS Letters | 2014 | 8 Pages |
Abstract
Biological functions of only some plant transcriptional repressors are known owing to the lack of knockout lines or unclear phenotypes because of redundancy. Here we show that strong viral activation domain VP16 fusion to the transcriptional repressor FLOWERING LOCUS C reversed its function and caused a stronger phenotype than that of the multiple-knockout line of redundant genes, suggesting the potential of this technique to identify transcription factor function that cannot be detected in a single-knockout line. Loss-of-function of transcriptional coactivator Mediator25 did not affect VP16 activity despite their in vivo interaction, suggesting the existence of other key mechanism(s) in plants.
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Agricultural and Biological Sciences
Plant Science
Authors
Sumire Fujiwara, Shingo Sakamoto, Keiko Kigoshi, Kaoru Suzuki, Masaru Ohme-Takagi,