Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870400 | FEBS Letters | 2014 | 5 Pages |
Abstract
Rare Caenorhabditis elegans males arise when sex chromosome non-disjunction occurs during meiosis in self-fertilizing hermaphrodites. Non-disjunction is a relatively rare event, and males are typically observed at a frequency of less than one in five hundred wild-type animals. Males are required for genetic crosses and phenotypic analysis, yet current methods to generate large numbers of males can be cumbersome. Here, we identify RNAi reagents (dsRNA-expressing bacteria) with improved effectiveness for eliciting males. Specifically, we used RNAi to systematically reduce the expression of over two hundred genes with meiotic chromosome segregation functions, and we identified a set of RNAi reagents that robustly and reproducibly elicited male progeny.
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Authors
Lisa Timmons, Hayley Luna, Jordan Martinez, Zachary Moore, Vaishnavi Nagarajan, Juliana Metichecchia Kemege, Nadeem Asad,