Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870401 | FEBS Letters | 2014 | 9 Pages |
Abstract
Catsper is a Ca2+permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription start sites and three functional Sox-binding sites were identified in the Catsper1 promoter. Interestingly, transcription factors Sox5 and Sox9 caused a significant increase in transactivation of the Catsper1 promoter in heterologous systems, and chromatin immunoprecipitation assays showed that both transcription factors interact with the Catsper1 promoter in vivo. These results provide new insights into the molecular mechanisms that control Catsper channel expression.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Minerva Mata-Rocha, Javier Hernández-Sánchez, Gabriel Guarneros, Elsa de la Chesnaye, Ana A. Sánchez-Tusié, Claudia L. Treviño, Ricardo Felix, Norma Oviedo,