| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10870415 | FEBS Letters | 2014 | 10 Pages |
Abstract
Polyphenol oxidases are involved in aurone biosynthesis but the gene responsible for 4-deoxyaurone formation in Asteraceae was so far unknown. Three novel full-length cDNA sequences were isolated from Coreopsis grandiflora with sizes of 1.80Â kb (cgAUS1) and 1.85Â kb (cgAUS2a, 2b), encoding for proteins of 68-69Â kDa, respectively. cgAUS1 is preferably expressed in young petals indicating a specific role in pigment formation. The 58.9Â kDa AUS1 holoproenzyme, was recombinantly expressed in E. coli and purified to homogeneity. The enzyme shows only diphenolase activity, catalyzing the conversion of chalcones to aurones and was characterized by SDS-PAGE and shot-gun type nanoUHPLC-ESI-MS/MS.
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Authors
Cornelia Kaintz, Christian Molitor, Jana Thill, Ioannis Kampatsikas, Claudia Michael, Heidi Halbwirth, Annette Rompel,