Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870425 | FEBS Letters | 2014 | 8 Pages |
Abstract
The interaction between HIV-1 integrase and LEDGF/P75 has been validated as a target for anti-HIV drug development. Based on the crystal structure of integrase in complex with LEDGF/P75, a library containing 80 thousand natural compounds was filtered with virtual screening. 11 hits were selected for cell based assays. One compound, 3-(1,3-benzothiazol-2-yl)-8-{[bis(2-hydroxyethyl)amino]methyl}-7-hydroxy-2H-chromen-2-one (D719) inhibited integrase nuclear translocation in cell imaging. The binding mode of D719 was analyzed with molecular simulation. The anti-HIV activity of D719 was assayed by measuring the p24 antigen production in acute infection. The structure characteristics of D719 may provide valuable information for integrase inhibitor design.
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Authors
Wan-Gang Gu, Xuan Zhang, Denis Tsz-Ming Ip, Liu-Meng Yang, Yong-Tang Zheng, David Chi-Cheong Wan,