Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870545 | FEBS Letters | 2014 | 7 Pages |
Abstract
To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at â free, 100 nM, 10 μM, and 1 mM Ca2+ by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ motif. With increasing [Ca2+], the affinity of the CSL-IQ interaction gradually decreased, and the affinity of the CSL-PreIQ binding gradually increased. The results suggest that CSL may bind with both the IQ and PreIQ motifs of the Cav1.2 channel in different Ca2+-dependent manners.
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Authors
Wei Sun, Rui Feng, Huiyuan Hu, Feng Guo, Qinghua Gao, Dongxue Shao, Dandan Yin, Hongmei Wang, Xuefei Sun, Meimi Zhao, Etsuko Minobe, Yingxian Sun, Guangyu Jiao, Masaki Kameyama, Liying Hao,