Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870596 | FEBS Letters | 2014 | 5 Pages |
Abstract
We developed an adenovirus-based CRISPR/Cas9 system for gene editing in vivo. In the liver, we demonstrated that the system could reach the level of tissue-specific gene knockout, resulting in phenotypic changes. Given the wide spectrum of cell types susceptible to adenoviral infection, and the fact that adenoviral genome rarely integrates into its host cell genome, we believe the adenovirus-based CRISPR/Cas9 system will find applications in a variety of experimental settings.
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Authors
Ranran Cheng, Jin Peng, Yonghong Yan, Peili Cao, Jiewei Wang, Chen Qiu, Lichun Tang, Di Liu, Li Tang, Jianping Jin, Xingxu Huang, Fuchu He, Pumin Zhang,