Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870723 | FEBS Letters | 2014 | 7 Pages |
Abstract
Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism as we have quantified the endogenous levels of N-acylglycines, N-acyldopamines, N-acylethanolamines, and primary fatty acid amides by LC/QTOF-MS. Growth of D. melanogaster on media supplemented with [1-13C]-palmitate lead to a family of 13C-palmitate-labeled fatty acid amides in the fly heads. The [1-13C]-palmitate feeding studies provide insight into the biosynthesis of the fatty acid amides.
Keywords
N-acylethanolaminePfamTICD. melanogasterFAAHCOANAEPAMPALPHMAdenosine TriphosphateATPFatty acid amide hydrolaseModel systemBiosynthetic pathwayEICDrosophila melanogasterpeptidylglycine α-amidating monooxygenasehigh performance liquid chromatographyHPLCextracted ion chromatogramtotal ion chromatogramcoenzyme A
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Authors
Kristen A. Jeffries, Daniel R. Dempsey, Anita L. Behari, Ryan L. Anderson, David J. Merkler,