Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870729 | FEBS Letters | 2014 | 7 Pages |
Abstract
Alternative splicing (AS) increases the size of the transcriptome and proteome to enhance the physiological capacity of cells. We demonstrate surface enhanced Raman spectroscopy (SERS) in combination with a DNA hybridization analytical platform to identify and quantify AS genes in plants. AS in AtDCL2 and AtPTB2 were investigated using non-fluorescent Raman probes using a 'sandwich assay'. Utilizing Raman probes conjugated to gold nanoparticles we demonstrate the recognition of RNA sequences specific to AtDCL2 and AtPTB2 splice junction variants with detection sensitivity of up to 0.1Â fM.
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Ulhas S. Kadam, Burkhard Schulz, Joseph lrudayaraj,