Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870734 | FEBS Letters | 2014 | 7 Pages |
Abstract
DNA repair mechanisms are key components for the maintenance of the essential mitochondrial genome. Among them, base excision repair (BER) processes, dedicated in part to oxidative DNA damage, are individually well known in mitochondria. However, no large view of these systems in differential physiological conditions is available yet. Combining the use of pure mitochondrial fractions and a multiplexed oligonucleotide cleavage assay on a microarray, we demonstrated that a large range of glycosylase activities were present in Drosophila mitochondria. Most of them were quantitatively different from their nuclear counterpart. Moreover, these activities were modified during aging.
Keywords
8oxoGUngNth1THFNEIL1APE1BERODNCy3OGG1PDH8-oxo-7,8-dihydroguanine8-oxoguanine DNA glycosylaseUracil DNA glycosylaseROSUracilOligonucleotideTetrahydrofuranDNA repairbase excision repairThymine glycolMicroarrayAgingAP siteabasic siteCyanine 3DrosophilaMitochondriahypoxanthinepyruvate dehydrogenaseReactive oxygen species
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Authors
Isabelle Garreau-Balandier, Mathilde Lefebvre, Sophie Jacquard, Sylvain Caillat, Luis Cruz-Rodriguez, Layal Ishak, Virginie Agier, Frédéric Morel, Philippe Lachaume, Pascal Dubessay, Sylvie Sauvaigo, Serge Alziari, Patrick Vernet,