| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10870739 | FEBS Letters | 2014 | 9 Pages |
Abstract
MicroRNA (miRNA) regulates expression of protein coding genes and has been implicated in diverse cellular processes including neuronal differentiation, cell growth and death. To identify the role of miRNA in neuronal differentiation, SH-SY5Y and IMR-32 cells were treated with dopamine cocktail and retinoic acid to induce differentiation. Detection of miRNAs in differentiated cells revealed that expression of many miRNAs was altered significantly. Among the altered miRNAs, human brain expressed miR-432 induced neurite projections, arrested cells in G0-G1, reduced cell proliferation and could significantly repress NESTIN/NES, RCOR1/COREST and MECP2. Our results reveal that miR-432 regulate neuronal differentiation of human neuroblastoma cells.
Keywords
nESRetinoic acid receptor alphaPTOTUBB3RARαCNR1MAP2Mecp2DAPI3′-UTR3′-untranslated region4′,6-diamidino-2-phenylindoleBDNFmicro RNAtyrosine hydroxylaseRetinoic acidNeurite outgrowthSH-SY5Y cellsbrain-derived neurotropic factorMiRNAnestincannabinoid receptor type 1methyl CpG binding protein 2Microtubule associated protein 2ChATcholine acetyltransferase
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Authors
Eashita Das, Nitai Pada Bhattacharyya,