Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870810 | FEBS Letters | 2013 | 6 Pages |
Abstract
A fungal galectin from Agrocybe cylindracea (ACG) exhibits broad binding specificity for β-galactose-containing glycans. We determined the crystal structures of wild-type ACG and the N46A mutant, with and without glycan ligands. From these structures and a saccharide-binding analysis of the N46A mutant, we revealed that a conformational change of a unique insertion sequence containing Asn46 provides two binding modes for ACG, and thereby confers broad binding specificity. We propose that the unique sequence provides these two distinct glycan-binding modes by an induced-fit mechanism.
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Authors
Naoyuki Kuwabara, Dan Hu, Hiroaki Tateno, Hisayoshi Makyio, Jun Hirabayashi, Ryuichi Kato,