Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10870900 | FEBS Letters | 2013 | 6 Pages |
Abstract
We have engineered a self-processing RNA, derived from the hairpin ribozyme that runs through a cascade of cleavage and ligation reactions thereby changing its topology. The first two cleavage events leave the resulting RNA with a 5â²-OH group and a 2â²,3â²-cyclic phosphate. Thus, upon refolding, intramolecular ligation delivers a cyclic species. In addition, we demonstrate formation of concatemers resulting from multiple intermolecular ligations. Our results demonstrate the potential of RNA for self-supported topology changes and support the suggestion of 2â²,3â²-cyclic phosphates being suitable activated building blocks for reversible phosphodiester bond formation in the RNA world.
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Authors
Sonja Petkovic, Sabine Müller,