| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10870923 | FEBS Letters | 2013 | 6 Pages |
Abstract
Pyrrolysyl-tRNA synthetase (PylRS) is a class IIc aminoacyl-tRNA synthetase that is related to phenylalanyl-tRNA synthetase (PheRS). Genetic selection provided PylRS variants with a broad range of specificity for diverse non-canonical amino acids (ncAAs). One variant is a specific phenylalanine-incorporating enzyme. Structural models of the PylRSamino acid complex show that the small pocket size and Ï-interaction play an important role in specific recognition of Phe and the engineered PylRS active site resembles that of Escherichia coli PheRS.
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Authors
Jae-hyeong Ko, Yane-Shih Wang, Akiyoshi Nakamura, Li-Tao Guo, Dieter Söll, Takuya Umehara,