Vangl2, the planner cell polarity protein, is complexed with postsynaptic density protein PSD-95

Vangl is a component of the non-canonical Wnt/planar cell polarity pathway, which is implicated in various cell polarity functions. However, little is known about its synaptic localization in neurons. Here, we show that Vangl1 and Vangl2 are expressed in adult rat neurons, where they are tightly associated with the postsynaptic density (PSD) fraction. Vangl2 forms a complex with PSD-95 through direct binding. Furthermore, the C-terminal PDZ-binding motif of Vangl2 is required for localization to dendritic spines. These results suggest that Vangl2 is a new component of the PSD that forms a complex with PSD-95 in the adult brain.