Recombinant expression of soluble murine prion protein for C-terminal modification

Article ID	Journal	Published Year	Pages	File Type
10871005	FEBS Letters	2013	6 Pages	PDF

Abstract

âº Generation of soluble PrP fusion proteins with DnaK and intein, respectively. âº Expression of up to 15Â mg/l of soluble cellular prion proteins in Escherichia coli. âº C-terminal modification of cellular prion proteins without any refolding.

Keywords

IPTG ASP GSH CBD GSSG NaOAc SBD PMSF GPi SrtA MESNA HDP NBD RML ASPM 4-Chloro-7-nitrobenzofurazan Sodium acetate isopropyl-β-d-thiogalactopyranoside Soluble expression substrate binding domain chitin binding domain phenylmethylsulfonyl fluoride Sortase A Semisynthesis Prion protein Disulfide bridge reduced glutathione oxidized glutathione glycosylphosphatidylinositol