Metamorphic protein IscU alternates conformations in the course of its role as the scaffold protein for iron-sulfur cluster biosynthesis and delivery

Article ID	Journal	Published Year	Pages	File Type
10871042	FEBS Letters	2013	8 Pages	PDF

Abstract

âº IscU is a metamorphic protein that converts between two folds: S and D. âº IscU adopts the D-state when interacting with IscS to pick up sulfur. âº Holo-IscU adopts the S-state, which is recognized by HscB. âº HscA:ATP does not bind IscU, but HscA:ADP binds the D-state of IscU. âº Conversion of IscU from the S- to the D-state leads to cluster release and delivery.

Keywords

ADP PLP ISCs IscU nuclear magnetic resonance Adenosine Triphosphate ATP adenosine diphosphate NMR Conformational equilibrium Protein–protein interaction Protein evolution iron–sulfur cluster Cysteine desulfurase pyridoxal 5′-phosphate