Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10871067 | FEBS Letters | 2013 | 6 Pages |
Abstract
Microtubules consisting of tubulin dimers play essential roles in various cellular functions. Investigating the structure-function relationship of tubulin dimers requires a method to prepare sufficient quantities of recombinant tubulin. To this end, we simultaneously expressed human α1- and β3-tubulin using a baculovirus-insect cell expression system that enabled the purification of 5 mg recombinant tubulin per litre of cell culture. The purified recombinant human tubulin could be polymerized into microtubules that glide on a kinesin-coated glass surface. The method provides a powerful tool for in vitro functional analyses of microtubules.
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Authors
Itsushi Minoura, You Hachikubo, Yoshihiko Yamakita, Hiroko Takazaki, Rie Ayukawa, Seiichi Uchimura, Etsuko Muto,