Effects of orexin A on proliferation, survival, apoptosis and differentiation of 3T3-L1 preadipocytes into mature adipocytes

Article ID	Journal	Published Year	Pages	File Type
10871157	FEBS Letters	2012	8 Pages	PDF

Abstract

âº Orexin A stimulates 3T3-L1 cells proliferation, and protects them from apoptosis. âº Mitogenic and antiapoptotic effects of orexin A are conferred via ERK1/2 signaling. âº Orexin A fails to enhance 3T3-L1 cells differentiation into mature adipocytes..

Keywords

Hprt IGF-1 SGM PPARγ FCS OXA DEXA ORO IBMX PI3K 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 3-isobutyl-1-methylxanthine 5-bromo-2′-deoxyuridine C/EBPα cAMP ERK1/2 MTT Cyclic adenosine monophosphate Adipocyte Akt orexin A BrdU Proliferation Apoptosis In vitro Dexamethasone Oil red O fetal calf serum insulin-like growth factor 1 Phosphatidylinositol 3-kinase Fibroblast SDM Hypoxanthine–guanine phosphoribosyltransferase CCAAT/enhancer binding protein alpha protein kinase B (PKB)extracellular signal-regulated kinase 1/2 Peroxisome proliferator-activated receptor gamma