Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10871159 | FEBS Letters | 2012 | 7 Pages |
Abstract
⺠We address the thermal unfolding of free and ligand-bound photoprotein obelin. ⺠Binding of coelenterazine and calcium considerably stabilizes obelin. ⺠Fluorescence and CD spectroscopy report formation of obelin molten globules. ⺠Apo-obelin is an ensemble of conformational states.
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Authors
Elena V. Eremeeva, Eugene S. Vysotski, Adrie H. Westphal, Carlo P.M. van Mierlo, Willem J.H. van Berkel,