| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10871268 | FEBS Letters | 2012 | 7 Pages |
Abstract
⺠Nonsense suppression by natural amino acids hinders genetic code expansion. ⺠Higher levels of near-cognate suppression found in opal versus amber codons. ⺠E. coli BL21 and MG1655 display higher background suppression than TOP10. ⺠PylRS/tRNAUCAPyl cannot outcompete Trp incorporation at opal codons. ⺠PylRS/tRNAUCCUPyl is outcompeted by Arg-tRNAArg in quadruplet decoding.
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Authors
Patrick O'Donoghue, Laure Prat, Ilka U. Heinemann, Jiqiang Ling, Keturah Odoi, Wenshe R. Liu, Dieter Söll,