| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10871387 | FEBS Letters | 2012 | 7 Pages |
Abstract
⺠We produced an improved system for production of recombinant phosphoproteins in Escherichia coli. ⺠Genome modification and strain engineering of E. coli enabled RF1 deletion. ⺠Single and double phosphoserine residues were incorporated into recombinant proteins at enhanced efficiency. ⺠Phosphoserine insertion at native UAG sites reduces cellular fitness and viability.
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Authors
Ilka U. Heinemann, Alexis J. Rovner, Hans R. Aerni, Svetlana Rogulina, Laura Cheng, William Olds, Jonathan T. Fischer, Dieter Söll, Farren J. Isaacs, Jesse Rinehart,