Article ID Journal Published Year Pages File Type
10871406 FEBS Letters 2012 6 Pages PDF
Abstract
► The single-chain variant of PvuII was structurally redesigned by circular permutation. ► DNA recognition and catalysis of the permuted variants of scPvuII were characterized. ► The permuted variants of scPvuII maintain the specific endonuclease activity.
Keywords
Related Topics
Life Sciences Agricultural and Biological Sciences Plant Science
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Redesigning the single-chain variant of the restriction endonuclease PvuII by circular permutation
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