Beyond hairballs: The use of quantitative mass spectrometry data to understand protein-protein interactions

Article ID	Journal	Published Year	Pages	File Type
10871654	FEBS Letters	2012	9 Pages	PDF

Abstract

âº Data generated by AP-MS is different from “binary” data and need to be better understood. âº Quantitative data in AP-MS can be used to understand complex organization. âº Quantitative AP-MS is poised to help understand interactome dynamics.

Keywords

SRM ORF MS1 AP-MS QconCAT Y2H NGF MS2 EGF STRIPAK cDNA Complementary DNA stable isotope labeling with amino acids in cell culture Tandem affinity purification Protein–protein interactions SILAC Interaction networks TAP epidermal growth factor nerve growth factor open reading frame Yeast two hybrid Single reaction monitoring Quantitative proteomics