| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10871756 | FEBS Letters | 2011 | 6 Pages |
Abstract
⺠We describe a method for metabolic labeling of palmitoylated Wnt and Hh. ⺠We use w-alkynyl palmitic acid and Click chemistry. ⺠The labeling does not interfere with Wnt or Hh signaling. ⺠Palmitoylated Wnt and Hh proteins are found in cellular membrane fractions. ⺠The method is useful for validating inhibitors of Wnt and Hh acyltransferases.
Keywords
PBStris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amineTBTAWntHEKFBSBPADMEMBSADMSOtris(2-carboxyethyl)phosphine hydrochloridebovine serum albuminHedgehogShhDimethyl sulfoxideTCEPfetal bovine serumsonic hedgehogPhosphate buffered salineDulbecco’s modified eagle’s mediumPalmitic acidProtein palmitoylationhuman embryonic kidney
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Authors
Xinxin Gao, Natalia Arenas-Ramirez, Suzie J. Scales, Rami N. Hannoush,