| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10871873 | FEBS Letters | 2011 | 7 Pages |
Abstract
⺠We examine the reported toxicity of TAP tagging proteins. ⺠TAP tagging does not generally cause loss of protein function. ⺠The MX marker cassettes are toxic to yeast at high copy numbers. ⺠Toxicity is due to the high copy presence of the heterologous TEF1 promoter. ⺠Genomic single copy integration of the MX marker and TEF1 promoter is not toxic.
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Authors
Roja Babazadeh, Soode Moghadas Jafari, Martin Zackrisson, Anders Blomberg, Stefan Hohmann, Jonas Warringer, Marcus Krantz,