Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10871941 | FEBS Letters | 2011 | 6 Pages |
Abstract
The nature of the ammonium import into prokaryotes has been controversial. A systems biological approach makes us hypothesize that AmtB-mediated import must be active for intracellular NH4+ concentrations to sustain growth. Revisiting experimental evidence, we find the permeability assays reporting passive NH3 import inconclusive. As an inevitable consequence of the proposed NH4+ transport, outward permeation of NH3 constitutes a futile cycle. We hypothesize that the regulatory protein GlnK is required to fine-tune the active transport of ammonium in order to limit futile cycling whilst enabling an intracellular ammonium level sufficient for the cell's nitrogen requirements.
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Authors
Fred C. Boogerd, Hongwu Ma, Frank J. Bruggeman, Wally C. van Heeswijk, Rodolfo GarcÃa-Contreras, Douwe Molenaar, Klaas Krab, Hans V. Westerhoff,