Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10871965 | FEBS Letters | 2011 | 6 Pages |
Abstract
The redox-midpoint potential of the FAD chromophore in the BLUF domain of anti-transcriptional regulator AppA from Rhodobacter sphaeroides equals â¼â260 mV relative to the calomel electrode. Altering the structure of its chromophore-binding pocket through site-directed mutagenesis brings this midpoint potential closer to that of free flavin in aqueous solution. The redox-midpoint potential of this BLUF domain is intermediate between those of LOV domains and Cryptochromes, which may rationalize the primary photochemistry observed in these three flavin-containing photoreceptor families. These results also imply that LOV domains, among the flavin-containing photosensory receptors, are least sensitive to intracellular chemical reduction in the dark.
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Authors
Jos C. Arents, Marcela Avila Perez, Johnny Hendriks, Klaas J. Hellingwerf,