Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10871997 | FEBS Letters | 2009 | 7 Pages |
Abstract
Novel synthetic collagen fibers, poly(PHG) made by polycondensation of Pro-Hyp-Gly, spontaneously assume polymeric structure with molecular weights greater than 105. Its application for biomaterials has been explored, but that for a platelet agonist has not been investigated. Poly(PHG)-induced platelet aggregation independently of thromboxane A2 and integrin α2β1. Poly(PHG)-induced tyrosine phosphorylation of glycoprotein VI (GPVI)-related molecules and failed to activate GPVI/FcRγ-deficient platelets. Binding of GPVI to poly(PHG) was confirmed by a surface plasmon resonance spectroscopy, suggesting that poly(PHG) activates platelets through GPVI. Poly(PHG) is an useful research tool to investigate GPVI-mediated signals and a substitute for collagen in platelet functional assays.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Osamu Inoue, Katsue Suzuki-Inoue, Daisuke Shinoda, Yasuto Umeda, Masazumi Uchino, Sin-ichi Takasaki, Yukio Ozaki,