Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872053 | FEBS Letters | 2010 | 8 Pages |
Abstract
The pleckstrin homology (PH) domain-containing protein casein kinase 2 interacting protein-1 (CKIP-1) plays an important role in regulation of bone formation and muscle differentiation. How CKIP-1 localization is determined remains largely unclear. We observed that isolated CKIP-1-PH domain was predominantly localized in the nucleus and the C-terminus of CKIP-1 counteracted its nuclear localization. The net charge of basic residues and a serine-rich motif within the PH domain plays a pivotal role in the localization switch of both full-length CKIP-1 and the isolated PH domain. We propose that the N-terminal PH domain and C-terminal auto-inhibitory region of CKIP-1 coordinate to determine its subcellular localization and the nucleus-plasma membrane shuttling.
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Authors
Shenli Xi, Yi Tie, Kefeng Lu, Minghua Zhang, Xiushan Yin, Jie Chen, Guichun Xing, Chunyan Tian, Xiaofei Zheng, Fuchu He, Lingqiang Zhang,