LOV2-linker-kinase phosphorylates LOV1-containing N-terminal polypeptide substrate via photoreaction of LOV2 in Arabidopsis phototropin1

Article ID	Journal	Published Year	Pages	File Type
10872070	FEBS Letters	2011	5 Pages	PDF

Abstract

âº LOV2-linker kinase of Arabidopsis phot1 was prepared. âº The kinase showed a similar photoreaction with LOV2-core. âº The kinase phosphorylated a substrate consisting of the N-terminal part of phot1. âº Amino acid substitution showed activation of the kinase via photoreaction of LOV2.

Keywords

CBB S390 SAXS PHOT1 PHOT GST GFP FMN Coomassie Brilliant Blue Phototropin t1/2 Arabidopsis thaliana Arabidopsis kinase domain flavin mononucleotide LOV Blue light Small-angle X-ray scattering green fluorescent protein Linker Kinase glutathione S-transferase