| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10872107 | FEBS Letters | 2011 | 7 Pages |
Abstract
Staphylokinase (SAK) forms a 1:1 stoichiometric complex with human plasmin (Pm) and switches its substrate specificity to generate a plasminogen (Pg) activator complex. Site-directed mutagenesis of SAKHis43 and SAKTyr44 demonstrated the crucial requirement of a positively charged and an aromatic residue, respectively, at these positions for optimal functioning of SAK-Pm activator complex. Molecular modeling studies further revealed the role of these residues in making cation-pi and pi-pi interactions with Trp215 of Pm and thus establishing the crucial intermolecular contacts within the active site cleft of the activator complex for the cofactor activity of SAK.
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Authors
Monika Dahiya, Satish Singh, Govindan Rajamohan, Deepti Sethi, Ashish Ashish, Kanak L. Dikshit,