Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872183 | FEBS Letters | 2009 | 7 Pages |
Abstract
Here, we tested the matrilin-3-dependent induction of osteoarthritis-associated genes in primary human chondrocytes. Matrilin stimulation leads to the induction of MMP1, MMP3, MMP13, COX-2, iNOS, IL-1β, TNFα, IL-6 and IL-8. Furthermore, we show the participation of ADAMTS4 and ADAMTS5 in the in vitro degradation of matrilin-3. We provide evidence for a matrilin-3-dependent feed-forward mechanism of matrix degradation, whereby proteolytically-released matrilin-3 induces pro-inflammatory cytokines as well as ADAMTS4 and -5 indirectly via IL-1β. ADAMTS4 and ADAMTS5, in turn, cleave matrilin-3 and may release more matrilin-3 from the matrix, which could lead to further release of pro-inflammatory cytokines and proteases in cartilage.
Keywords
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Authors
Andreas R. Klatt, Gabriele Klinger, Brigitte Paul-Klausch, Gertrud Kühn, Joerg H. Renno, Raimund Wagener, Mats Paulsson, Joachim Schmidt, Gebhart Malchau, Klaus Wielckens,