Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872334 | FEBS Letters | 2011 | 7 Pages |
Abstract
In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains.
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Authors
Carles Gil, Anton Falqués, Eduard Sarró, Roger Cubı´, Juan Blasi, José Aguilera, Emilio Itarte,