Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872405 | FEBS Letters | 2007 | 7 Pages |
Abstract
In Saccharomyces cerevisiae, one of two cytosolic lysine-tRNAs is partially imported into mitochondria. We demonstrate that three components of the ubiquitin/26S proteasome system (UPS), Rpn13p, Rpn8p and Doa1p interact with the imported tRNA and with the essential factor of its mitochondrial targeting, pre-Msk1p. Genetic and biochemical assays demonstrate that UPS plays a dual regulatory role, since the overall inhibition of cellular proteasome activity reduces tRNA import, while specific depletion of Rpn13p or Doa1p increases it. This result suggests a functional link between UPS and tRNA mitochondrial import in yeast and indicates on the existence of negative and positive import regulators.
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Authors
Irina Brandina, Alexandre Smirnov, Olga Kolesnikova, Nina Entelis, Igor A. Krasheninnikov, Robert P. Martin, Ivan Tarassov,