Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10872562 | FEBS Letters | 2007 | 7 Pages |
Abstract
In plant cells, the vacuole functions as a major calcium store. Although a calmodulin-regulated Ca2+-ATPase (ACA4) is known to be present in prevacuolar compartments, the presence of an ACA-type Ca2+-ATPase in the mature vacuole of a plant cell has not been verified. Here we provide evidence that ACA11 localizes to the vacuole membrane. ACA11 tagged with GFP was expressed in stable transgenic plants, and visualized in root cells and protoplasts by confocal microscopy. A Ca2+-ATPase function for ACA11 was confirmed by complementation of yeast mutants. A calmodulin binding domain was identified within the first 37 residues of the N-terminal autoinhibitory region.
Keywords
Related Topics
Life Sciences
Agricultural and Biological Sciences
Plant Science
Authors
Sang Min Lee, Ho Soo Kim, Hay Ju Han, Byeong Cheol Moon, Cha Young Kim, Jeffery F. Harper, Woo Sik Chung,